Journal: iScience

Article Title: Preclinical safety and efficacy of a therapeutic antibody that targets SARS-CoV-2 at the sotrovimab face but is escaped by Omicron

doi: 10.1016/j.isci.2023.106323

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Article Snippet: Anti-light-kappa-PE (clone IS11-24D5) , Miltenyi Biotec , Cat# 130-123-246; RRID: AB_2802012.

Techniques: Virus, Recombinant, Blocking Assay, Saline, Enzyme-linked Immunosorbent Assay, Membrane, Plasmid Preparation, Expressing, Software

Journal: Acta neuropathologica communications

Article Title: Molecular signatures of inherited and acquired sporadic late onset nemaline myopathies.

doi: 10.1186/s40478-023-01518-9

Figure Lengend Snippet: Fig. 4 Analysis of immunoglobulin expression and autoimmune markers in inherited and sporadic late-onset nemaline myopathies (A) Proteomic analysis showed an increased abundance of IgG heavy chains and both kappa and lambda light chains in nemaline rod areas in sporadic late-onset nemaline myopathy (SLONM) relative to inherited nemaline myopathies (iNM). There was also a trend towards an increased abundance of immunoglobulin chains in the nemaline rod areas of SLONM relative to rod-free areas. Striped bars indicate immunoglobulin chains that were only detected in one of the groups of samples compared *p < 0.05. (B) Immunofluorescent staining demonstrated accumulation of kappa light chains in atrophic fibers in 38% of SLONM biopsies (bottom row), but not in iNM (top row). Scale bar = 400 µm. (C) Immunoglobulin genes were also differentially expressed between SLONM and iNM. (D) Immunostaining for Major Histocompatibility Antigen-I (MHC-I) showed reactivity in SLONM biopsies, occurring predominantly in atrophic fibers (first panel, SLONM with monoclonal gammopathy; second panel, SLONM without monoclonal gammopathy). Biopsies from patients with inherited nemaline myopathy showed no MHC-I reactivity or occasional fibers faintly reactive for MHC-I (third panel, adult patient with ACTA1 nemaline myopathy). MHC-I reactivity was absent in healthy control muscle (fourth panel). Scale bar = 100 µm

Article Snippet: Frozen skeletal muscle sections (8 μm) were post-fixed in 4% paraformaldehyde for 5 min at room temperature prior to immunostaining with laminin (L0663, Sigma, dilution 1:250) and human kappa light chain (NBP269235, Novus Biologicals, Centennial, CO, dilution 1:200) antibodies as previously described [18].

Techniques: Expressing, Staining, Immunostaining, Control